Western Blot Protein Detection: Enzymatic vs. Fluorescence Methods

Western blotting is a popular technique in cell and molecular biology for detecting proteins. Historically, researchers used a chemiluminescent signal and darkrooms to detect binding. With advances in technology such as charge-coupled devices and new labels, other detection methods including fluorescence and chemifluorescence are becoming more common. Scientists must consider cost, equipment and reagent availability, and the specific detection parameters required before deciding on a detection method.

Download this article from Analytik Jena to learn about

• The major differences between enzymatic and fluorescence methods
• When to use chemiluminescent, colorimetric, fluorescent, and chemifluorescent detection