Accurate localization of mouse primary antibodies in mouse tissue is confounded by endogenous mouse immunoglobulins present in the tissue, that react with the anti-mouse secondary antibody reagent, causing off-target staining. In order to eliminate this background staining, reagents which block the endogenous mouse immunoglobulins and prevent non-specific staining are commonly used, and known as Mouse-on-Mouse or MOM kits.
Download this application note for a comparison of MOM IHC kits with various sample types.
