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eBOOK

Understanding and Optimizing PCR

Do you know how to get the most out of your PCR reactions?

 

 

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PCR is one of the most commonly used techniques in biological studies. However, its ubiquity belies its complexity. There are a lot of moving parts to every PCR experiment, including DNA template, primers, reagents, and cycling conditions. Each of these parts has a specific role and purpose. Difficulties with any of the reaction components affect PCR end-products in specific ways.

Download this eBook from The Scientist's Creative Services team and MilliporeSigma to explore

  • How to improve DNA template quality
  • The individual components of PCR reactions
  • Recognizing common PCR issues
  • Tips and tricks for troubleshooting PCR