PCR is one of the most commonly used techniques in biological studies. However, its ubiquity belies its complexity. There are a lot of moving parts to every PCR experiment, including DNA template, primers, reagents, and cycling conditions. Each of these parts has a specific role and purpose. Difficulties with any of the reaction components affect PCR end-products in specific ways.
Download this eBook from The Scientist's Creative Services team and MilliporeSigma to explore
- How to improve DNA template quality
- The individual components of PCR reactions
- Recognizing common PCR issues
- Tips and tricks for troubleshooting PCR
