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Understanding and Optimizing PCR

Do you know how to get the most out of your PCR reactions?




PCR is one of the most commonly used techniques in biological studies. However, its ubiquity belies its complexity. There are a lot of moving parts to every PCR experiment, including DNA template, primers, reagents, and cycling conditions. Each of these parts has a specific role and purpose. Difficulties with any of the reaction components affect PCR end-products in specific ways.

Download this eBook from The Scientist's Creative Services team and MilliporeSigma to explore

  • How to improve DNA template quality
  • The individual components of PCR reactions
  • Recognizing common PCR issues
  • Tips and tricks for troubleshooting PCR