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The Impact of the Claristep® Filtration System on Protein Recovery and Adsorption

Alexander Croon and Johannes Felix Buyel




Biopharmaceutical samples often contain insoluble particles like cell debris or protein aggregates from their biotechnological production processes. These particles can damage analytical instruments and are typically removed by centrifugation or filtration. While centrifugation can only separate based on differences in density, filtration can achieve absolute particle removal, making it preferable.

However, syringe filters usually have large sample dead volumes of about 100 μL, which is disadvantageous as only small sample volumes <200 μL are typically available in early phase small-scale experiments. Moreover, a mere <50 μL of sample can be sufficient for state-of-the-art analytical devices like (U)HPLC consoles.

If sample volumes are small, unspecific protein binding to the filter material can occur and either impair quantification or completely prevent sample analysis due to alterations in sample composition. In addition, the large numbers of samples required for applications like biopharmaceutical release testing analysis can be time and labor intensive if using regular single-use filters.

Download this application note to learn about how a novel multiplexed filtration device makes sample preparation rapid with negligible differences in protein loss!