Spatial Profiling of Immune Signatures in the Tumor Microenvironment

Single-cell technologies enable researchers to precisely analyze heterogeneous tissues to identify key therapeutic targets and biomarkers. In particular, studying target gene expression with spatial context is vital for understanding cellular organization and function within the context of the tissue. Researchers use RNAscope^TM in situ hybridization (ISH) for single molecule detection of RNA transcripts at sub-cellular and single-cell resolutions, including short RNA targets, splice variants, and miRNAs.

Download this application note from ACD, a Bio-Techne brand, to learn how to employ a multiplexed in situ transcriptomic approach for spatial mapping of target genes in highly heterogeneous tumor tissues using RNAscope ISH technology.