Library construction for Next Generation Sequencing (NGS) using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide variations in sample quality. Differences in formalin fixation methods, storage conditions, and age lead to crosslinked and/or degraded nucleic acid and inconsistent extraction yields. FFPE extraction and library construction methods must be carefully considered for target enrichment applications.
Download this application note from Twist Bioscience to discover a complete library preparation and target enrichment solution that generates ready-to-sequence multiplexed libraries directly from FFPE tissue of various qualities.
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