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Scanning Viral Genomes with 300mer Oligonucleotides

Researchers use long custom oligos to screen viral genome stability with an NGS workflow.




Complex RNA structures facilitate viral replication and block antiviral host factors. To identify the sequences giving rise to these structures and determine their function, researchers recently developed a next generation sequencing (NGS)-based screening workflow called Fate-seq. This technique utilizes custom 300mer oligonucleotides to scan across genomes in a high-throughput manner. Using Fate-seq, researchers screened genomic sequences long enough to identify these complex secondary structures in the SARS-CoV and SARS-CoV-2 genome.

Download this application note from Twist Bioscience to learn about

  • Custom oligonucleotides and the Fate-seq workflow
  • Identifying high-dimensional RNA structures with long oligos and NGS
  • How SARS viruses may evade host immune responses