Complex RNA structures facilitate viral replication and block antiviral host factors. To identify the sequences giving rise to these structures and determine their function, researchers recently developed a next generation sequencing (NGS)-based screening workflow called Fate-seq. This technique utilizes custom 300mer oligonucleotides to scan across genomes in a high-throughput manner. Using Fate-seq, researchers screened genomic sequences long enough to identify these complex secondary structures in the SARS-CoV and SARS-CoV-2 genome.
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