Reprobing Immunoassay Samples

Protein expression drives many biological and pathological processes. Identifying and quantifying changes in protein levels is therefore critical for understanding the underlying mechanisms behind health and disease. However, disease research is plagued by limited sample sizes, and the most common assays used to assess protein expression require relatively large amounts of sample to generate each data point.

Capillary-based immunoassays present a solution to this problem by offering highly reproducible quantitative data while consuming miniscule sample amounts. Capillary-based immunoassays can probe the same sample with multiple antibodies without disrupting epitope integrity.

Download this application note to explore how Jess^TM, a complete protein analysis solution from ProteinSimple, automates multiplexed protein detection and quantification.