Redesigning Molecular Cloning for Rapid Protein Synthesis

Cell-free expression systems for the production of recombinant proteins are game changers compared to traditional cloning methods. The cell-free system workflow removes the molecular cloning, transformation, and in vivo gene expression steps for greater yields of high-fidelity protein products. Using the cell-free approach, researchers can build high-throughput protein expression libraries for cytotoxic proteins that are difficult to synthesize due to their ill effects on cell growth and viability.

Download this application note from Integrated DNA Technologies to learn about

• Commercially available gBlocks™ gene fragments
• The advantages of cloning with linear DNA templates
• High-throughput recombinant protein screening