The main in vitro methods for immunologists are flow cytometry, PCR, and various forms of immunoassay. Although extremely powerful, these methodologies provide little insight into cell morphology or spatial interactions, and are not well suited to reporting on changes in biology over time.
Real-time live-cell analysis is the quantification of the behavior of living cells over significant periods of time using time-lapse imaging. It is ideally suited for the investigation of both general immune cell biology—proliferation, differentiation, and cell health—and specialized functions such as phagocytosis, chemotaxis, cytolysis, and viral infection. It can be used with all types of immune cells, and is suitable for monocultures, co-cultures, and more advanced models such as 3D cell cultures.
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