Hit-to-lead characterization is an enormous task during drug development. After high throughput or virtual screening of small molecule inhibitors that target enzymes, researchers must analyze and prioritize hundreds of hits. They often use a series of biochemical assays that measure enzyme products to ultimately find the right compound for further development.
Download this guide from BellBrook Labs to learn about these critical aspects of hit prioritization:
- Biochemical assay considerations
- Dose response
- Hit confirmation
- Triaging
- Mechanism of action studies
