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Preparing Sensitive and Accurate NGS Libraries

Reliably detect rare variants with unique molecular identifiers.



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When preparing next-generation sequencing (NGS) libraries, PCR amplification of targets can introduce bias and propagate processing errors. Researchers hoping to detect rare variants in their NGS libraries need methods with increased sensitivity, greater accuracy, and lower limits of detection. Unique molecular identifiers (UMIs) add molecular barcodes to DNA and RNA, grouping fragments into read families to eliminate false-positive reads and increase detection sensitivity.

Download this white paper from Canopy Biosciences to learn about

  • Tagging NGS libraries with UMIs
  • Detecting rare variants with RareSeqTM—an NGS method that uses UMIs
  • The RareSeq workflow