When cellular oxygen levels change, the first response is metabolic, evoking responses that rapidly translate into physiological changes. The central event for metabolic activity in most cells is mitochondrial oxidative phosphorylation, important for both ATP production and oxygen consumption.
Treating and manipulating cells during experimentation within atmospheres containing aberrant levels of oxygen has direct effects on oxidative phosphorylation, thus perturbating all cellular events. In our attempts to detect and understand subtle cellular reactions, attention needs to be paid to the basic conditions of the cells in order to establish solid grounds for translational results.
Download this application note from The Baker Company to learn about:
- How oxygen concentrations impact cell metabolism
- The difference in oxygen level between experimental and physiological conditions
- The impact of reactive oxygen species production on cell physiology
- How to control experimental oxygen levels
