To produce recombinant proteins, scientists introduce recombinant DNA containing genes of interest into host production cells. Prokaryotic cells cannot produce fully functional, properly folded multiunit proteins or generate post-translational modifications. While mammalian cells can overcome these obstacles, they need to be cultured in physiologically-relevant conditions that are difficult to achieve with conventional 2-D culture techniques. 3-D culture systems such as hollow-fiber bioreactors (HFBRs) can recapitulate in vivo conditions with minimal user intervention.
Download this application note from FiberCell Systems to discover how HFBRs generate difficult-to-produce proteins, boost yields, and improve product consistency.