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Optimizing Library Preparation for Tumor Samples

Discover how to detect low-frequency, tumor-associated variants from low-yield, low-quality tumor samples.




Comprehensive tumor profiling using next generation sequencing (NGS) technologies is key to discovering new, targetable biomarkers for cancer research. However, converting tumor samples into NGS libraries can be challenging. Tumor samples may be formalin-fixed and paraffin-embedded (FFPE) to preserve tissue structure, which reduces tumor DNA quality. Similarly, cell-free DNA (cfDNA) samples collected from liquid biopsies often yield low quantities of tumor-derived DNA. Careful library conversion of low-quality, low-quantity DNA samples is critical to sequencing success.

Download this application note from IDT to discover

  • The benefits of combining the xGenTM Prism DNA Library Prep Kit with IDT xGen hybridization reagents for the sensitive and accurate detection of low-frequency tumor variants
  • How researchers successfully detect tumor-associated variants in matched formalin-fixed, paraffin-embedded, and cell-free DNA samples.


For research use only. Unless otherwise agreed to in writing, IDT does not intend these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.