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Next-Generation Sequencing (NGS) Sample Preparation

Best practices for optimizing sample homogenization in NGS nucleic acid extraction.




Homogenization frees nucleic acids from intact animal tissues, food, and fecal samples for sequencing analysis. Uniform homogenization is critical for extracting sufficient nucleic acid yields that produce high-quality next-generation sequencing (NGS) data. While there are many methods for homogenizing cells and tissues, mechanical lysis and bead-beating technology repeatedly demonstrate high-quality, high-yield nucleic acid extraction, making these approaches preferable for downstream NGS analysis.

Download this white paper from Bertin to receive six optimized homogenization protocols for NGS workflows:

  • Robust and unbiased microbial RNA extraction workflow
  • Microbiota analysis of fermented milk samples with NGS
  • Transcriptomic analysis of cheese ripening microbial communities with dual RNA
  • MicroRNA expression profiling of individual rat hypothalamic nuclei
  • DNA extraction from frozen tumor samples
  • Optimized protocol for viral metagenomics studies on fecal samples