Microfluidic diffusional sizing (MDS) is a method for measuring protein interactions – with affinity and absolute size reported in the same test. In it, fluorescently labeled protein samples are passed through a diffusion chamber alongside a protein-free auxiliary stream. Proteins diffuse from the sample stream to the auxiliary stream based on size, with small proteins moving quickly and larger ones migrating more slowly.
MDS offers several advantages when it comes to measuring protein size and determining binding affinity:
Watch this webinar from Fluidic Analytics to learn more about MDS, how it can be used to measure not only protein size and binding affinity, but also stoichiometry and protein conformation in protein-protein, protein-antibody, and protein-aptamer interactions!