Microfluidic Diffusional Sizing (MDS) uses a novel fluidics-based approach for measuring protein size in a more natural environment that decreases the likelihood of conformational protein changes. Using a diffusion chamber, proteins from a sample move according to size into an adjacent protein-free stream. Small proteins diffuse more quickly into the stream than larger proteins.
In this webinar sponsored by Fluidic Analytics, Sean Devenish discusses the advantages of using microfluidic diffusional sizing to study difficult proteins, such as intrinsically disordered proteins, multi-protein complexes, or aggregated fibrils. Only labeled proteins are detected and measured with MDS, which offers the added advantage of studying the effect of different buffers on protein size and interaction in complex heterogenous mixtures.
Watch this webinar from Fluidic Analytics to learn more about