Moving cell culture plates from the incubator to the workbench and back exposes cells to variable oxygen levels, which rapidly affect gene expression and cell morphology. Ideally, researchers maintain their cultures at constant physiological oxygen concentrations, even while performing experiments. Closed bench culture systems mimic in vivo conditions by creating controlled environments where parameters such as oxygen remain consistent. Researchers combine live cell imaging and cell assays with these workstations to analyze their cultures in physiological conditions.
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