Improved reagents and careful donor design boost HDR rates for genome engineering.
Homology-directed repair (HDR) mechanisms enable researchers to introduce precise genome-level changes using CRISPR. However, HDR competes with other repair mechanisms, such as non-homologous end joining (NHEJ), and low HDR rates are a persistent problem. There are several ways to promote HDR over these other repair mechanisms, including reagent choice, Cas9 gRNA selection, and HDR donor template design.
Watch this webinar from Integrated DNA Technologies to explore how CRISPR experiment optimization can promote HDR.
