Production of Exosomes in a Hollow Fiber Bioreactor

John J.S. Cadwell


Exosomes are small lipid membrane vesicles (80-120 nm) which export protein and miRNA outside cells into the circulatory system. Exosomes have been implicated in tissue repair, as well as tumor initiation, growth, progression, metastasis, and drug resistance.

Our understanding of exosomes has been impeded by their low availability and difficulty in producing large quantities for experimentation. The most common production method employs large numbers of T flasks. However, the composition and activity of exosomes reflect the physiologic state of the cells when secreting them, and this method is far from physiologic or relevant to in vivo conditions.

Hollow fiber bioreactors (HFBR) offer significant advantages over flask culture, especially for the collection and concentration exosomes under physiologic cell culture conditions. HFBRs are the most efficient way to culture large numbers of cells for exosome production as they grow in vivo.

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