Yeast is a preferred microorganism for large-scale protein production in biotechnology and synthetic biology applications. Researchers engineer new yeast strains using random mutagenesis to maximize protein yield. However, undefined mutations often disrupt physiological processes and weaken the yeast. To circumvent this problem, researchers use an automated, multiplexed CRISPR-editing platform to precisely target the yeast genome and improve production yield without affecting the health and physiology of the strain.
Download this application note from Inscripta to learn about a genome-wide CRISPR library construction workflow using the OnyxTM platform and its applications in engineering yeast strains.
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