The Gibson Assembly® method is a molecular cloning procedure that can join two or more DNA fragments without restriction enzyme digestion or compatible restriction sites. This innovative approach has been cited in over 1,200 peer-reviewed publications since its creation in 2008.
Due to its many advantages over traditional cloning methods, Gibson Assembly cloning has become the preferred method for cloning DNA into plasmids and bacterial artificial chromosomes (BAC) in many laboratories. By designing DNA fragments with overlapping ends, the Gibson Assembly method can create DNA constructs containing multiple inserts, using virtually any vector, in a single round of cloning.
Seamless assembly using the Gibson Assembly method can be readily applied to both routine cloning and large and complex cloning projects.
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