Droplet digital PCR (ddPCR) enables easy multiplexing of numerous targets within each fluorescent channel. Multiplexing with ddPCR allows for improved sensitivity and precision, as well as increased low-level detection in limited sample types like liquid biopsies. This is especially useful for increasing the sensitivity when detecting somatic mutations, edits, viruses, and other very rare targets.
Download this white paper to learn about two strategies for measuring multiple targets simultaneously by creating a unique endpoint fluorescence for each target:
Download this white paper to learn about two strategies for measuring multiple targets simultaneously by creating a unique endpoint fluorescence for each target:
- Amplitude-based multiplexing, which varies primer and/or probe concentrations
- In probe-mixing multiplexing, where probes are mixed at desired concentrations to place targets in a defined position on a 2-D plot
