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eBOOK

Early Discoveries in Molecular Cloning

Learn about key methods and milestones that made DNA cloning possible


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Scientists accept bacterial competency, restriction enzyme digestion, recombinant DNA technology, and DNA assembly as simple, fundamental laboratory tools, but these methods were hard won by early trailblazing scientists. Learn how these early scientists experimented with the building blocks of molecular biology to shape the field of molecular cloning.

Download this eBook to learn how

  • A hunch about sticky ends revealed the principles of recombinant DNA technology
  • Isolating endonucleases became a restriction enzyme enterprise
  • Scientists first made bacteria competent to take up DNA
  • A moonlit drive inspired PCR
  • The race to create the first synthetic cell led the discovery of Gibson Assembly