Directed Evolution of Novel Biocatalysts

Directed evolution improves the function of proteins and enzymes and has applications in organic synthesis and biotechnology. However, multiple shortcomings prevent researchers from using this approach to its full potential. Because of problems in common PCR-based mutagenesis techniques, there is an urgent need to develop efficient screening and selection tools for biocatalyst improvement. Scientists can mitigate PCR difficulties by using solid-phase DNA synthesis technology for combinatorial variant library generation. This approach reduces codon bias, avoids sequence errors, and identification of novel mutants with improved properties.

Download this application note from Twist Bioscience to learn how scientists are using high fidelity solid-phase chemical gene synthesis on silicon chips with efficient gene assembly for protein discovery.