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Detecting Proteins in Their Native Environments

A quantitative immunofluorescence assay combines the best aspects of traditional Western blots and ELISAs.



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When performing traditional Western blots, researchers must process their samples to separate proteins from the rest of their cellular environment. As an alternative, the In-Cell WesternTM Assay enables scientists to identify proteins in cells grown in microplates, combining the specificity of Western blotting with the replicability and throughput of ELISAs.

Download this guide from LI-COR to learn about the In-Cell Western Assay.

  • Uses of In-Cell Western Assays
  • Developing In-Cell Western Assay
  • Example protocols by cell type
  • Step-by-step guides for each facet of the experiment