New genome editing tools such as the CRISPR system have greatly expanded the ability to modify genes, including creating single nucleotide polymorphisms (SNPs) and small indels, in different models. But the time and resources required to screen large numbers of cells to identify gene edited samples is a considerable bottleneck.
Researchers have options when it comes to CRISPR screening. One of these options is high-resolution melting (HRM). HRM measures temperature variations with high precision as the DNA strands melt, thus detecting mutations through subsequent melt curve analysis. HRM limits the amount of sequencing required at each step, reducing the amount of resources required for both screening and sample preparation.
In this infographic from Canon BioMedical, find out:
- How CRISPR works
- A typical CRISPR workflow involving HRM screening
- 5 tips for successful CRISPR screening
- Your options when it comes to CRISPR screening, and how HRM stacks up
