Functional genetic screening using the CRISPR system is a powerful tool for interrogating gene function at scale. However, existing pre-defined CRISPR sgRNA libraries constrain scientists implementing this technology, especially in the context of focused screens targeting specific subsets of genes. Using Twist Oligo Pools with a simple bioinformatics and wet-lab workflow, scientists can generate multiple individual custom sgRNA libraries from a single oligo pool to facilitate multiple focused CRISPR screens.
Download this application note from Twist Biosciences to discover how
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