Combining Live Cell Imaging with Cellular Impedance to Monitor
Apoptotic Cell Death in Real Time

Apoptosis (also known as programmed cell death) is an evolutionarily conserved process that enables multicellular organisms to eliminate cells without triggering an inflammatory response. Once triggered by intrinsic or extrinsic stimuli, apoptosis causes a broad array of biochemical and morphological modifications, the net result of which is the fragmentation of the cell into apoptotic bodies that are rapidly phagocytosed by macrophages. Although apoptosis can be tracked using various biomarker-based assays, these typically require multiple manual handling steps and only yield endpoint measurements.

Download this application note to learn how the ACEA xCELLigence RTCA eSight system can:

• Continuously monitor drug-mediated apoptosis over the course of multiple days
• Quantitatively track early (cell shrinkage) to late (fragmentation) apoptotic events with high analytical sensitivity
• Capture live cell images in brightfield and three fluorescence channels (red, green, and blue), providing an orthogonal readout of the apoptosis process