In many biomedical research fields, such as cancer research, drug development, and tissue engineering, living cells are used to perform a variety of assays. Mammalian cell cultures are an essential tool in biology because they facilitate the rapid growth and proliferation of different cell types for experimental analysis and experimentation.
In order to successfully work with mammalian cell lines, they must be grown under specific controlled environmental and nutritional conditions. In addition, their growth must be monitored at regular intervals to ensure consistency. When a cell line reaches approximately 80% confluence (surface area coverage) within the vessel, the cells must be subcultured to ensure continued cell health and proper growth.
