Cell confluency is a key parameter for all cell biologists, as it is the beginning of all other cell culture-related experiments, including  transfection, cell-based assays, and cell culture-quality control. Accurate cell confluency calculations are important for obtaining maximum transfection efficiency and avoiding waste. Despite this, many cell biologists use subjective ways to estimate cell culture confluency. They look at the space covered by cells in a culture flask, or manually count a small aliquot of trypan blue-stained cells using a hemacytometer. 
"Scratch" assays are widely used to measure cell migration in vitro. These assays involve creating a "scratch" in a cell monolayer and imaging cell migration into the newly formed scratch at regular intervals. The technique is widely applied to studying cell migration, drug effects, angiogenesis, cell-cell interactions, wound healing, and metastasis.
In this white paper from Bertin Technologies, learn how to use a cell imager system to:
  • Directly and accurately capture and calculate cell confluency
  • Use cell confluency data to determine cell migration rate