--- title: "Best Practices: Calculating Cell Confluency" image: https://offers.the-scientist.com/hubfs/downloads/TS/TS_Bertin_PPL/Bertin-1200x630.png --- # [The Scientist](http://www.the-scientist.com/)   ## **BEST PRACTICES: CALCULATING CELL CONFLUENCY** ![](https://offers.the-scientist.com/hs-fs/hubfs/The%20Scientist%20Scripts%20and%20Images/TS%20E-blast%20Images/BD_line-horz.jpg?width=600&name=BD_line-horz.jpg) ![Bertin-500x250](https://offers.the-scientist.com/hs-fs/hubfs/downloads/TS/TS_Bertin_PPL/Bertin-500x250.png?width=500&height=250&name=Bertin-500x250.png) Cell confluency is a key parameter for all cell biologists, as it is the beginning of all other cell culture-related experiments, including  transfection, cell-based assays, and cell culture-quality control. Accurate cell confluency calculations are important for obtaining maximum transfection efficiency and avoiding waste. Despite this, many cell biologists use subjective ways to estimate cell culture confluency. They look at the space covered by cells in a culture flask, or manually count a small aliquot of trypan blue-stained cells using a hemacytometer.    **In this white paper from Bertin Technologies, learn how to use a cell imager system to:** - Directly and accurately capture and calculate cell confluency - Use cell confluency data to determine cell migration rate   [![Share on Facebook](https://static.hubspot.com/final/img/common/icons/social/facebook-24x24.png)](http://www.facebook.com/share.php?u=https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Dfacebook) [![Share on LinkedIn](https://static.hubspot.com/final/img/common/icons/social/linkedin-24x24.png)](http://www.linkedin.com/shareArticle?mini=true&url=https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Dlinkedin) [![Share on Twitter](https://static.hubspot.com/final/img/common/icons/social/twitter-24x24.png)](https://twitter.com/intent/tweet?original_referer=https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Dtwitter&url=https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Dtwitter&source=tweetbutton&text=Best%20Practices%3A%20Calculating%20Cell%20Confluency) [![Share on Email](https://static.hubspot.com/final/img/common/icons/social/email-24x24.png)](mailto:?subject=Check%20out%20https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Demail%20&body=Check%20out%20https%3A%2F%2Foffers.the-scientist.com%2Fbest-practices-calculating-cell-confluency-bertin-technologies%3Futm_medium%3Dsocial%26utm_source%3Demail) *The Scientist*needs the contact information you provide us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy](https://www.the-scientist.com/privacy-policy).   [![](https://offers.the-scientist.com/hs-fs/hubfs/Images/TS/The%20Scientist%20Logo_white%20on%20222222.png?width=250&name=The%20Scientist%20Logo_white%20on%20222222.png)](https://www.the-scientist.com) The Scientist c/o LabX Media Group 1000 N West Street, Suite 1200, Wilmington, Delaware, United States,19801 Toll Free: 888.788.0328 | Phone: 705.528.6888 Email: [privacy@the-scientist.com](mailto:privacy@the-scientist.com) © 1986-2026[The Scientist](http://the-scientist.com/)[Privacy Policy](https://www.the-scientist.com/privacy-policy)