Conventional western blotting requires labor-intensive and time-consuming protocols that utilize multiple hands-on steps, which increase the risk of user error and data variability. This renders conventional western blot data only semi-quantitative, creating a need for an automated, reproducible, and quantifiable protein analysis method. New technologies eliminate common protein analysis workflow challenges by automating separation, incubation, and detection with picogram-level sensitivity. Furthermore, capillary-based technology allows researchers to re-probe each sample while maintaining protein integrity.
Download this application note from ProteinSimple, a Bio-Techne brand, to discover how to detect multiple targets per sample, quantify total protein, and attain picogram-level sensitivity.