The Scientist Logo_white

Accelerating Discovery with Single Cell CRISPR Screens

Researchers combine guide RNA identification and single cell transcriptomic analysis with direct capture technology.

10x Logo NEW 2021


473x300 Laptop on F6F6F6

Single cell CRISPR screens, such as Perturb-seq, combine pooled CRISPR screens with single cell transcriptomics to enhance phenotypic profiling of manipulated cells. However, available methods for single cell CRISPR screens face technical and practical limitations that limit a screen’s combinatorial power and scalability. Researchers recently developed direct capture Perturb-seq to overcome these limitations and to enable scientists to perform complex CRISPR screens with combinatorial gene manipulations.

Download this research snapshot from 10x Genomics to discover

  • How direct capture Perturb-seq works
  • How this technology permits combinatorial CRISPR perturbations
  • How direct capture compares to indirect guide capture technologies