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A Guide to Comprehensive Epigenetic Analysis

Technological advances help scientists detect genome-wide DNA methylation and hydroxymethylation levels in parallel.





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Next-generation sequencing technologies have helped scientists establish causal relationships between genetic aberrations and disease. Researchers have expanded sequencing technologies to detect epigenetic modifications and study their links to disease through epigenome-wide association studies (EWAS). Such studies typically use bisulfite sequencing to detect DNA methylation, but this technology cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), two important DNA-based epigenetic modifications.

Download this application note from Tecan to discover

  • How to detect DNA methylation and hydroxymethylation with the TrueMethyl oxBS Module
  • Methods for performing comprehensive epigenome-wide association studies that analyze both 5mC and 5hmC levels
  • How researchers applied TrueMethyl oxBS to detect an abnormal epigenetic signature in multiple system atrophy (MSA) patients