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A Comprehensive Guide to Multiplex Western Blots

Researchers use fluorescent western blotting to quantify multiple targets on a single blot.



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Scientists regularly perform western blots to analyze protein expression and determine the activity of signaling pathways. When researchers analyze multiple proteins from the same protein lysate, they must strip their protein membrane and reprobe it with a new antibody or run multiple protein gels. Neither option allows scientists to reliably compare expression levels. To analyze multiple proteins on a single blot, scientists have optimized fluorescent western blot technology.

Download this technical note from Thermo Fisher Scientific to learn

  • Tips and tricks to minimize background fluorescence
  • How to select compatible fluorophores and optimize their detection
  • How to troubleshoot common problems with fluorescent western blots