Label-free binding assays allow scientists to study how biological molecules interact with each other in conditions that closely match the molecules’ native biological conformations. Several methods exist to examine molecular interactions in near real time, including bio-layer interferometry, surface plasmon resonance, isothermal titration calorimetry, and microscale thermophoresis. Because key experimental parameters, such as throughput, affinity range, and sensitivity, differ between methods, scientists must carefully weigh these factors when deciding on the biosensor platform to suit their application and workflow needs.
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