If DNA is the blueprint of life, then proteins comprise the edifices themselves. Naturally, the study of proteins – their structures, abundance, function, and interactions – has proven integral to our understanding of biology. Protein-centered research is pivotal to human health and disease, and protein-based products are consumed commercially, used in academic research, and serve as biopharmaceuticals.
To effectively study proteins or manufacture protein-based products, researchers need to be able to detect, characterize, and quantify the protein(s) of interest. The actual steps involved in protein extraction and preparation protocols will depend on a host of factors, but all protein preparation protocols strive to optimize two main parameters: yield and purity.
Download this eBook from The Scientist and MilliporeSigma to learn about:
⋅ Common challenges to protein preparation protocols
⋅ A typical protein isolation workflow
⋅ Considerations when working with challenging samples
⋅ Confirming intact protein extraction