.png?width=500&name=Merck_500x250%20(1).png)
Extracellular vesicles (EVs) are membrane-derived structures that include exosomes, microvesicles, and apoptotic bodies. Exosomes, in particular, have been shown to transfer molecules between cells and can transfer signals between cells. Exosomes are released under normal physiological conditions, but are also believed to serve as mediators in the pathogenesis of neurological, vascular, hematological, and autoimmune diseases, as well as cancer.
Quantifying and characterizing EVs in a reproducible and reliable manner is difficult due to their small size (exosomes range from 30 to 100 nm in diameter). Although EV analysis can be performed using high-magnification microscopy, this technique has a very low throughput. Attempts to analyze EVs using traditional PMT-based flow cytometers has been hampered by the limit of detection of such small particles and their low refractive index.
Download this application note from Merck to learn how the Amnis® time delay integration (TDI) image capturing system allows the CellStream™ flow cytometer to perform high-throughput flow cytometry with high sensitivity to submicron particles!
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the United States and Canada.
