Generating Site-Saturation Mutagenesis Libraries

 Quickly and efficiently designing both NNN and NNK libraries

Creating mutations at specific DNA positions has proven to be an invaluable research tool for elucidating protein structure and function. A site-saturation variant library consists of a pool of constructs containing degenerate codons encoding the 20 standard amino acids across a random or targeted region. The variability of such libraries can span a region of a protein of interest, or the mutations can be site-directed, targeted at a particular amino acid site or sites. 
The BioXpTM 3200 System constructs DNA fragments and plasmids for a variety of downstream applications, including site-directed mutagenesis studies. Learn how to rapidly construct high-variability and low-error site-saturation variant libraries in this application note from Synthetic Genomics!
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