Creating mutations at specific DNA positions has proven to be an invaluable research tool for elucidating protein structure and function. A site-saturation variant library consists of a pool of constructs containing degenerate codons encoding the 20 standard amino acids across a random or targeted region. The variability of such libraries can span a region of a protein of interest, or the mutations can be site-directed, targeted at a particular amino acid site or sites.
The BioXpTM 3200 System constructs DNA fragments and plasmids for a variety of downstream applications, including site-directed mutagenesis studies. Learn how to rapidly construct high-variability and low-error site-saturation variant libraries in this application note from Synthetic Genomics!
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