Preparing samples for flow cytometry commonly involves centrifuging, pelleting, and washing cells to remove excess unbound fluorescent marker. This process is inherently stressful and can potentially alter cell biology.
Laminar flow washing can quickly and effectively remove excess fluorescent marker without the need for centrifugation or pelleting, while maintaining up to 95% cell retention when applied to suspensions. Laminar flow-based protocols can also be automated, minimizing the hands-on-time required to 3-4 minutes versus the 20-40 minutes needed with standard centrifugation-based methods.
Find out more about how laminar flow-based techniques stack up against conventional centrifugation-based flow cytometry methods and how to incorporate laminar flow washing into your flow cytometry workflows with this information package from Curiox BioSystems!