BEST PRACTICES FOR SAMPLE PREPARATION AND LIPID EXTRACTION FROM VARIOUS SAMPLES

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Lipids play a structural role in organisms, forming cellular and organelle membranes. Recent studies have also shown their role as first and second messengers in signal transduction. Given that they have been implicated in widespread pathologies such as cancer and inflammation, lipids have become a major concern for the scientific community.

Lipid extraction for analysis can be challenging due to their structure and composition in various biological materials. Different types of lipids, such as complex organic compounds, fats, oils, waxes, and even hormones, exist in living cell membranes. Although numerous lipid extraction methods exist today, a vast majority of them start with cell or tissue homogenization. Bead beating has been reported to be one of the most effective and rapid cell and tissue disruption methods, increasing lipid extraction efficiency at lower costs.

Download this white paper from Bertin Technologies to learn about:
  • Drosophila homogenization for lipid analysis
  • Extraction of lipid hydroperoxides from tumor and non-tumor rat tissues
  • High-throughput lipid extraction for the analysis of human brain lipids